Furthermore, recommendations are given on how remaining data gaps and research needs can be addressed. In light of the deadlines established in the EU Cosmetics Directive for cessation of animal testing for cosmetic ingredients, a COLIPA scientific meeting was held in Brussels on 30th January, 2008 to review the use of alternative approaches and to set up a decision-tree approach for their integration into tiered testing strategies for hazard and safety assessment of cosmetic ingredients and their use in products. Despite the numerous pride parades and LGBT community activists, it’s still hard to come out for regular. Let’s cover the topic of homosexual relationships and find out just how much gay you are. Although not formally validated, several other in vitro models have been used for over a decade by the cosmetics industry as valuable tools in a weight of evidence approach for the safety assessment of ingredients and finished products. If you here for this Am I gay quiz, it means that you’ve been exploring your sexuality and would like to get answers to some questions. Even though, to date, no single in vitro assay has been validated as a full replacement for the rabbit Draize eye test, organotypic assays are accepted for specific and limited regulatory purposes. Extensive research has lead to the development of several assays, some of which have undergone formal validation. The need for alternative approaches to replace the in vivo rabbit Draize eye test for evaluation of eye irritation of cosmetic ingredients has been recognised by the cosmetics industry for many years.